human fibroblast-like synoviocytes: hfls  (Cell Applications Inc)

Journal: JACC: Basic to Translational Science

Article Title: HDL Regulates TGFβ-Receptor Lipid Raft Partitioning, Restoring Contractile Features of Cholesterol-Loaded Vascular Smooth Muscle Cells

doi: 10.1016/j.jacbts.2025.101461

Figure Lengend Snippet: Contractile Gene Expression Is Downregulated in Chol-Loaded hVSMCs (A, B) Human vascular smooth muscle cells (hVSMCs) were treated with cholesterol (Chol) (5 μg/mL) or 0.2% bovine serum albumin (control [CT]) for 24 hours and 48 hours and gene expression of Acta2 , Tagln , Cnn1, Myocd, and Srf were determined by quantitative polymerase chain reaction. (C) hVSMCs were treated with Chol (5 μg/mL) or 0.2% bovine serum albumin (CT) for 24 hours and protein expression of α–smooth muscle actin (α-SMA) and CNN1 were determined by Western blotting (representative blots shown). Densitometry showing the (D) α-SMA and (E) CNN1 band intensities normalized to GAPDH. For data analysis, unpaired Student’s t -testing was performed for comparing the means of 2 groups. For 2 or more independent groups, 1-way analysis of variance followed by Dunnett post hoc test was performed. A P value of ≤0.05 was considered significant. Data are presented as the mean ± SEM of 3 independent experiments, and P values are as indicated (∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001).

Article Snippet: Human coronary artery smooth muscle cells (referred to as hVSMC) were purchased from Cell Applications and maintained in complete medium (#311-500) as provided by the vendor. hVSMCs were used within 8 passages for all experiments.

Techniques: Gene Expression, Control, Real-time Polymerase Chain Reaction, Expressing, Western Blot